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. 2020 Jun 15;12(6):2760–2768.

Figure 2.

Figure 2

miR-101a knock down protect H9c2 cell form oxidative injury. (A) qPCR was performed to evaluate the expression level of miR-101a. (B) Flow cytometry was applied to investigate the apoptosis rate. (C) Western blot was used to evaluate the expression of apoptotic proteins such as bax, cleaved caspase3 and bcl-2. (D) CK, (E) LDH, (F) ROS was evaluated by commercial kits. (G) JC-1 staining was performed to detect the mitochondrial membrane potential (MMP) of H9c2 cell. Data are expressed as mean ± SD; n=8 *P<0.05.