Figure 4.

BCL2L11 knock down reversed the effect of miR-101a inhibition on myocardiomyocyte oxdative stress injury. (A, B) Flow cytometry was applied to investigate the apoptosis rate after different kinds of treatment. (C) Western blot was used to evaluate the expression of apoptotic proteins such as bax, cleaved caspase3 and bcl-2. (D) CK, (E) ROS and (F) LDH was evaluated by commercial kits. (G) JC-1 staining was performed to detect the mitochondrial membrane potential (MMP) of H9c2 cell. Data are expressed as mean ± SD; n=8 *P<0.05.