Figure 2.

circMMP11 is upregulated in BC cells and tissue and correlated with poor clinical characteristics of BC. A. Heatmap of the top 10 aberrantly expressed circRNAs. B. circMMP11 is located on Chr.22 and consisted of the eighth exon of MMP11. C. Splicing site of circMMP11 verified by DNA sequencing. D. The divergent primer designed for circMMP11 and the convergent primer for MMP11 at different exons. E, F. circMMP11 and linear MMP11 were both upregulated in BC cell lines. G. circMMP11 expression level was higher in BC tissues (BC) than in adjacent nontumor tissues (ADJ) detected by qRT-PCR (n = 113). H. Expression level of MMP11 was higher in BC tissue than in normal tissue which was analyzed through Gepia2. I. Higher level of MMP11 was associated with poorer survival of patients with BC. Data were shown as the mean ± SD, *P < 0.05.