Figure 3.

Silencing of circMMP11 inhibits BC cell proliferation, migration and invasion. A. Schematic representation of the sites of the siRNA specific to the back-splice junction of circMMP11. B. qRT-PCR analysis of circMMP11 and MMP11 mRNA expression after treatment with three siRNAs. siRNA3 has the best silencing efficacy. C-E. CCK-8 assay showed that circMMP11 knockdown significantly inhibited cell proliferation of T47D, MDA-MB-231 and Hs 578T. F, G. circMMP11 knockdown reduced the colony formation ability of these cells. H, I. Transwell assay without Matrigel revealed that cell migration was significantly suppressed in circMMP11 silenced group. J, K. Transwell assay with Matrigel revealed that knockdown of circMMP11 significantly reduced cell invasive capability. Data were shown as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.