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. 2020 Jun 15;12(6):2585–2599.

Figure 4.

Figure 4

circMMP11 acts as a sponge for miR-1204. A. Approximately 70% or more of circMMP11 was located in cytoplasm of BC cell lines. B. RNA interaction network predicted by multiple database. C-E. qRT-PCR analysis of predicted miRNAs in BC cell lines transfected with control (si-NC) or circMMP11 siRNA (si-circMMP11). miR-1204 was screened out as the one candidate. F. Binding sites for circMMP11 and miR-1204. G. miR-1204 expression level was lower in BC cell lines than in normal cells. H. Dual luciferase reporter assay of HEK 293T cells cotransfected with miR-NC and miR-1204 mimics and a luciferase reporter containing circMMP11-wt or mutant constructs with mutated miR-1204 binding sites (circMMP11-mut). I, J. After transfection of miR-1204 mimics, the expression of circMMP11 was suppressed. Data were shown as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.