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. 2020 Jun 15;12(6):2585–2599.

Figure 5.

Figure 5

MMP11 is directly targeted by miR-1204 and indirectly regulated by circMMP11. A. Venn diagram of the four candidate mRNAs predicted through multiple databases and microarray. B. MMP11 was screened out as potential target by qRT-PCR analysis in BC cell lines transfected with control (mimic-NC) or miR-1204 mimics. C. Silencing of circMMP11 could also inhibit the expression of linear MMP11. D. qRT-PCR analysis of linear MMP11 in clinical samples (n = 113). MMP11 was upregulated in BC tissue than in adjacent normal tissue. E. The expression level of circMMP11 was positively correlated with that of MMP11. F. Binding sites for linear MMP11 and miR-1204. G. Dual luciferase reporter assay of HEK 293T cells cotransfected with miR-NC and miR-1204 mimics and a luciferase reporter with full-length MMP11 3’-UTR containing target sites for miR-1204 (MMP11 wt) or mutant constructs with mutated miR-1204 binding sites (MMP11 mut). Data were shown as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ADJ: adjacent normal tissue.