Skip to main content
. 2020 Jun 15;12(6):2738–2748.

Figure 3.

Figure 3

MiR-205-3p confirmed to be a direct target of MALAT1. A. The putative miR-205-3p binding sequence of the wild type and mutation sequence of MALAT1. B. Luciferase activity was detected in different groups. Wide type (MALAT1-WT) and mutant MALAT1 (MALAT1-MUT) were co-transfected with miR-205-3p mimic or control mimic (miR-NC) into bEnd.3 cells. C. The expression of miR-205-3p was detected in different groups by using RT-PCR. D. Cellular lysates from End.3 cells were used for RIP with an Ago2 antibody and IgG antibody. The expression of MALAT1 and miR-205-3p were detected by qRT-PCR. Data were represented as mean ± SD. *P<0.05, **P<0.01, and ***P<0.001.