Figure 1.

Effect of combination drugs on morphological features of HSCs. Combined everolimus and N-acetylcysteine treatment preserved morphological features of HSCs. A. Morphology of drug treated HSCs upon culturing, as visualized by phase contrast microscopy. HSCs are spontaneously activated as a result of growth in untreated and treated groups. B. Lipid droplets in the cytoplasm of HSCs, incubated with BODIPY. Green fluorescence indicates lipid droplets and blue indicates the nucleus. A considerable number of lipid droplets remain in the combination treatment group, until day 14. Morphological observation conducted at 200 times magnification.