Figure 2.

Effect of combination drugs on fibrosis related genes. Expression of α-SMA (A) and collagen (B) mRNA in drug treated HSCs for the indicated period, using RT-PCR. Combination treatment on HSCs markedly inhibits the hepatic fibrosis related genes. Representative agarose gel showing mRNA expression of fibrogenic markers in HSCs. Quantification of expression of α-SMA and collagen, relative to glyceraldehyde 3-phosphate dehydrogenase, from day 3 to day 14. The ratio between each sample and glyceraldehyde 3-phosphate dehydrogenase was calculated to normalize for initial variation in sample concentration and as a control for reaction efficiency. Values are shown as mean ± SEM. *P < 0.05, **P < 0.001.