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. 2020 Jun 15;12(6):2538–2553.

Figure 4.

Figure 4

LINC01619 served as a competing endogenous LncRNA (ceRNA) to sponge miR-129-5p in NSCLC. A. LINC01619 WT and MUT fragments containing the binding site for miR-129-5p was designed. B. Luciferase reporter assay indicated that miR-129-5p was directly inhibited by LINC01619. C and D. RIP assay revealed that LINC01619 was binding to miR-129-5p. E. RNA FISH experiment showed that LINC01619 and miR-129-5p were mainly colocalized in the cytoplasm. F. LINC01619 up-regulation reduced miR-129-5p expression in SPCA1 cells, whereas LINC01619 down-regulation elevated miR-129-5p expression in A549 cells. G. miR-129-5p expression was reduced in NSCLC tissues than that in normal tissues. H. In NSCLC tissues, prominently negative correlation between LINC01619 and miR-129-5p expression level was found. **P < 0.01.