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. 2020 Jun 15;12(6):2570–2584.

Figure 2.

Figure 2

MiR-125b regulated autophagy and the function of the epithelial barrier. A. qPCR measurement of miR-125b in cells treated with PBS (Control), Derp1, or cells transfected with miR-125b mimics or inhibitor. B. Western blot analysis of LC3 and Beclin-1 in cells treated with PBS (Control), cells transfected with miR-125b mimics or inhibitor, or cells transfected with miR-125b mimics and treated with 3-MA (miR-125b mimics+3-MA). C. qPCR measurement of Occludin, Claudin-1, ZO-1, and JAM-A in cells treated with PBS (Control), cells transfected with miR-125b mimics or inhibitor, or cells transfected with miR-125b mimics and treated with 3-MA (miR-125b mimics+3-MA). D. Western blot analysis of Occludin, Claudin-1, ZO-1, and JAM-A in cells treated with PBS (Control), cells transfected with miR-125b mimics or inhibitor, or cells transfected with miR-125b mimics and treated with 3-MA (miR-125b mimics+3-MA). Data were shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.01.