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. 2020 Jun 15;12(6):2570–2584.

Figure 3.

Figure 3

MiR-125b regulated autophagy of epithelial cells via FoxP3. A. Binding site of miR-125b on the 3’UTR of FoxP3. B. Dual luciferase reporter assay on FoxP3-wild type cells (WT) and FoxP3-mutant cells (MUT) transfected with miR-125b mimics, non-specific miRNA (mimics NC), or not transfected (Control). C. qPCR measurement of FoxP3 in cells transfected with miR-125b mimics or inhibitor. D. Western blot analysis of FoxP3 in cells transfected with miR-125b mimics or inhibitor and untransfected control cells. E. Western blot analysis of LC-3 II/I and Beclin-1 in cells transfected with miR-125b inhibitor, both miR-125b inhibitor and sh-FoxP3 (miR-125b inhibitor+sh-FoxP3), or cells transfected with both miR-125b mimics and pcDNA3.1-FoxP3 (miR-125b mimics+pcDNA3.1-FoxP3). Data were shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01.