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. 2020 Jun 15;12(6):2570–2584.

Figure 6.

Figure 6

Validation of the regulatory effect of the CXCR4/miR-125b/FoxP3 axis on autophagy and the epithelial barrier in an in vivo AR model. A. Western blot analysis of LC3 II/I and Beclin-1 in animals treated with normal saline, Derp1, both Derp1 and AMD3100, or both Derp1 and 3-MA. B. qPCR measurement of Occludin, Claudin-1, ZO-1, and JAM-A in animals treated with normal saline, Derp1, both Derp1 and AMD3100, or both Derp1 and 3-MA. C. Western blot analysis of Occludin, Claudin-1, ZO-1, and JAM-A in animals treated with normal saline, Derp1, both Derp1 and AMD3100, or both Derp1 and 3-MA. D. FD4 permeability assay in animals treated with normal saline, Derp1, both Derp1 and AMD3100, or both Derp1 and 3-MA. Data were shown as mean ± SD from three independent experiments. *P < 0.05, **P < 0.01.