Table 1.
Primers for quantitative real-time PCR
| Gene | Primers | Sequences (5’-3’) |
|---|---|---|
| CXCR4-h | Forward | CTCCTCTTTGTCATCACGCTTCC |
| Reverse | GGATGAGGACACTGCTGTAGAG | |
| miR-125b-h | Forward | CCTGAGACCCTAACTTG |
| Reverse | GAACATGTCTGCGTATCTC | |
| FoxP3-h | Forward | GGCACAATGTCTCCTCCAGAGA |
| Reverse | CAGATGAAGCCTTGGTCAGTGC | |
| Occludin-h | Forward | ATGGCAAAGTGAATGACAAGCGG |
| Reverse | CTGTAACGAGGCTGCCTGAAGT | |
| Claudin-1-h | Forward | GTCTTTGACTCCTTGCTGAATCTG |
| Reverse | CACCTCATCGTCTTCCAAGCAC | |
| ZO-1-h | Forward | GTCCAGAATCTCGGAAAAGTGCC |
| Reverse | CTTTCAGCGCACCATACCAACC | |
| JAM-A-h | Forward | GTGAAGTTGTCCTGTGCCTACTC |
| Reverse | ACCAGTTGGCAAGAAGGTCACC | |
| β-actin-h | Forward | CTCCATCATGAAGTGTGACGTT |
| Reverse | ATCTCCTTCTGCATCCTGTCAG | |
| U6 snRNA-h | Forward | CGCAAGGATGACACGCAAATTC |
| Reverse | GTGCAGGGTCCGAGGT | |
| CXCR4-r | Forward | GGATGGTGGTGTTCCAGTTCTCCCCACGTAATACGGTAGC |
| Reverse | TCCCCACGTAATACGGTAGC | |
| miR-125b-r | Forward | ACACTCCAGCTGGGTCCCTGAGACCCTAACTT |
| Reverse | TGTCGTGGAGTCGGCAATTC | |
| FoxP3-r | Forward | CCCAAATTCCTGCCTGTGCTTA |
| Reverse | GCTGAAGACGTGTGCATCCTAT | |
| Occludin-r | Forward | CAGACCACTATGAAACCGACTA |
| Reverse | TCTCCAGCAACCAGCATC | |
| Claudin-1-r | Forward | GGGACAACATCGTGACTGCT |
| Reverse | CCACTAATGTCGCCAGACCTG | |
| ZO-1-r | Forward | CACGATGCTCAGAGACGAAGG |
| Reverse | TTCTACATATGGAAGTTGGGGATC | |
| JAM-A-r | Forward | ATCCCACAACAGGAGAGCTGCTCGGCTATAGGCAAACCAG |
| Reverse | CTCGGCTATAGGCAAACCAG | |
| β-actin-r | Forward | GGGAAATCGTGCGTGACATT |
| Reverse | CAGGAAGGAAGGCTGGAAGA | |
| U6 snRNA-r | Forward | GCTTCGGCAGCACATATACTAAAATGGCAGCACATATACTAAAAT |
| Reverse | CGCTTCAGAATTTGCGTGTCAT |
h, Human; r, Rat; CXCR4, C-X-C motif chemokine receptor type 4; ZO-1, zonula occludens-1; JAM-A, junctional adhesion molecule-A.