Figure 4.

The numbers of CD8⁺ T cells were reduced in the M2c infusion groups. (A) Immunofluorescence microscopic analysis for identification of CD8 positive T cells 7 days following liver transplantation with original magnifications of ×400. CD8 was immunostained with Cy3 (left graph) and nucleus were stained with DAPI (middle graph). The right graph shows the merge of the Cy3 and the DAPI. Scale bar in right lower corner represents 25 µm. (B) The numbers of the CD8⁺ T cells in (A) were counted and quantitatively compared. The CD8⁺ T cells were significantly reduced in the M2c infusion groups. No significant differences were observed between the M0 and the PBS group. Data were analyzed by one-way ANOVA, followed by post hoc test of Tukey’s (n = 5 for each group). Data are presented as the mean ± SD. ***P < 0.001 vs M0 group.