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. 2020 Jun 15;12(6):2939–2955.

Figure 2.

Figure 2

Effects of lncRNA KRT16P2 on LSCC cells. (A) Small interfering RNAs targeting KRT16P2 (si1-KRT16P2 and si2-KRT16P2) were obtained and KRT16P2 silence was generated in TU212 and TU686 cells by the transfection of si1-KRT16P2 or si2-KRT16P2. The transfection efficiency was verified by real-time PCR; si2-KRT16P2 was selected for further experiments because of better transfection efficiency. Next, TU212 and TU686 cells were transfected with si2-KRT16P2 and examined for (B) cell viability by CCK-8 assay; Blank is the background of OD value. (C) Invasion capacity by Transwell assay; (D) Migration capacity by Wound healing assay; (E) The protein levels of ki-67, PCNA, pro-Caspase 3, cleaved-Caspase 3, p-AKT, and AKT by Immunoblotting. ANOVA followed by Turkey’s multiple comparison test.