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. 2020 Jun 15;12(6):2939–2955.

Figure 5.

Figure 5

Effects of miR-1294 on LSCC cells. (A) TU212 and TU686 cells were divided into four groups: NC mimics (negative control for miR-1294 mimics), miR-1294 mimics (to conduct miR-1294 overexpression), NC inhibitor (negative control for miR-1294 inhibitor), and miR-1294 inhibitor (to conduct miR-1294 inhibition). Cells were non-transfected or transfected with miR-1294/NC mimics or miR-1294/NC inhibitor and examined for cell viability by CCK-8 assay. Blank is the background of OD value. Next, Cells were non-transfected or transfected with miR-1294/NC mimics or miR-1294/NC inhibitor and examined for (B) invasion capacity by Transwell assay; (C) Migration capacity by Wound healing assay; (D) The protein levels of ki-67, PCNA, pro-Caspase 3, cleaved-Caspase 3, p-AKT, and AKT by Immunoblotting. ANOVA followed by Turkey’s multiple comparison test.