Figure 2.

BMP4 induced autophagy in HCC cell lines. A, B. BMP4 treatment increased LC3-II conversion and Beclin1 expression, while reduced p62 expression. The LC3-II/GAPDH ratio was significantly increased in the BMP4 groups (P < 0.001). C. After treatment with BMP4 for 24 h, an increased accumulation of fluorescent cells with LC3 dots as compared with the control groups was observed (P < 0.01, respectively). Data are shown as mean ± SD and from three independent experiments. **: P < 0.01, ***: P < 0.001.