TABLE 1.
Formulation and proximate composition of experimental diets (% dry matter).
| Ingredients | A | B | C | D |
| Fish meala | 25.00 | 15.00 | 15.00 | 15.00 |
| Soybean meala | 25.00 | 25.00 | 25.00 | 25.00 |
| Peanut meala | 12.00 | 12.00 | 12.00 | 12.00 |
| Soy protein concentrateb | 0.00 | 9.00 | 9.00 | 9.00 |
| Wheat flour | 23.20 | 21.04 | 20.79 | 20.54 |
| Beer yeasta | 2.00 | 2.00 | 2.00 | 2.00 |
| Shrimp meala | 2.00 | 2.00 | 2.00 | 2.00 |
| Chicken meala | 3.00 | 3.00 | 3.00 | 3.00 |
| Fish oila | 1.00 | 2.00 | 2.00 | 2.00 |
| Soy oil | 1.50 | 1.50 | 1.00 | 0.50 |
| Schizochytrium limacinumc | 0.00 | 0.00 | 0.75 | 1.50 |
| Choline chloride (50%) | 0.20 | 0.25 | 0.25 | 0.25 |
| Soy lecithina | 1.00 | 1.00 | 1.00 | 1.00 |
| Vitamin and mineral mixtured | 2.00 | 2.00 | 2.00 | 2.00 |
| Monocalcium phosphate | 1.00 | 2.00 | 2.00 | 2.00 |
| Vc | 0.10 | 0.10 | 0.10 | 0.10 |
| Met-Mete | 0.00 | 0.20 | 0.20 | 0.20 |
| L-lysine monohydrochloridef | 0.00 | 0.25 | 0.25 | 0.25 |
| Threoninef | 0.00 | 0.05 | 0.05 | 0.05 |
| Glycinef | 0.00 | 0.25 | 0.25 | 0.25 |
| Alaninef | 0.00 | 0.15 | 0.15 | 0.15 |
| GABAf | 0.00 | 0.02 | 0.02 | 0.02 |
| Taurinef | 0.00 | 0.10 | 0.10 | 0.10 |
| Ornithinef | 0.00 | 0.02 | 0.02 | 0.02 |
| Phytaseg | 0.00 | 0.04 | 0.04 | 0.04 |
| Vitamin B12 | 0.00 | 0.001 | 0.001 | 0.001 |
| Vitamin B6 | 0.00 | 0.001 | 0.001 | 0.001 |
| ZnSO4⋅7H2O | 0.00 | 0.02 | 0.02 | 0.02 |
| FeSO4.7H20 | 0.00 | 0.02 | 0.02 | 0.02 |
| KI | 0.00 | 0.0001 | 0.0001 | 0.0001 |
| Folate | 0.00 | 0.0001 | 0.0001 | 0.0001 |
| Na2SeO3 | 0.00 | 0.00006 | 0.00006 | 0.00006 |
| CMC | 1.00 | 1.00 | 1.00 | 1.00 |
| Proximate composition (%) | ||||
| Dry matter | 90.16 | 89.35 | 89.19 | 90.23 |
| Crude protein | 41.35 | 42.22 | 42.34 | 42.04 |
| Crude lipid | 6.70 | 6.35 | 6.43 | 6.32 |
aSupplied by Haida Feed Corporation, Guangzhou, China. bSupplied by Wilmar, Beijing, China. cSupplied by Kingdomway, Xiamen, China, protein, 17.5%; lipids, 48.6%; DHA, 19.4%; EPA, 5.8%; palmitic acid, 7.3%; others, 3%. dSupplied by Ashare, Guangzhou, China. Composition of vitamin and mineral mixture (kg–1 of mixture): vitamin A, 250,000 IU; riboflavin, 750 mg; pyridoxine HCL, 500 mg; cyanocobalamin, 1 mg; thiamin, 500 mg; menadione, 250 mg; folic acid, 125 mg; biotin, 10 mg; a-tocopherol, 3,750 mg; myo-inositol, 2,500 mg; calcium pantothenate, 1,250 mg; nicotinic acid, 2,000 mg; vitamin D3, 45,000 IU; vitamin C, 7,000 mg, Zn, 4,000 mg; K, 22,500 mg; I, 200 mg; NaCl, 2.6 g; Cu, 500 mg; Co, 50 mg; FeSO4, 200 mg; Mg, 3,000 mg; Se, 10 mg. eSupplied by Evonik, Beijing, China. fSupplied by Aladdin Industrial Corporation, Shanghai, China. gSupplied by Yiduoli Biotechnology Co., Ltd, Zhuhai, China.