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. 2020 Jun 30;11:1368. doi: 10.3389/fmicb.2020.01368

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Copyright © 2020 Bidossi, Bottagisio, Savadori and De Vecchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal laser scanning microscopy analysis of synovial fluid infected by Staphylococcus aureus 4. (a) Sample stained with SYTO 9 for cellular biomass (green, left panel), BOBO-3 for eDNA (light blue, central panel), and WGA stain for GlcNAc containing polysaccharides (red, right panel). (b) Sample stained with SYTO 9 for cellular biomass (green, left panel); SYPRO-Ruby staining shows the proteins contained in the biofilm matrix (yellow, right panel).