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. 2020 Jun 30;11:1368. doi: 10.3389/fmicb.2020.01368

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Copyright © 2020 Bidossi, Bottagisio, Savadori and De Vecchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection of matrix components in in vitro assays. (a) Staphylococcus aureus 1; left panel, SYTO 9 for cellular biomass; central panel, BOBO-3 for eDNA staining; and right panel, WGA stain for GlcNAc containing polysaccharides. (b) S. aureus 1; left panel, SYTO 9 for cellular biomass; right panel, SYPRO-Ruby staining. (c) Detection of fibrin incorporation in S. aureus 2 biofilm-like aggregate; left panel, SYTO 9 for nucleic acids (green); right panel, α-fibrin antibody (gray).