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. 2020 Jun 30;8:685. doi: 10.3389/fbioe.2020.00685

FIGURE 5.

FIGURE 5

The co-immunoprecipitation was used to verify interactions between the full-length Cry1Ab/c protein and 12 endogenous proteins. Protein extracts (Input) were immunoprecipitated with GFP-trap beads (IP) and resolved by SDS-PAGE. The immunoblots shown were developed with anti-GFP antibody to detect the target endogenous protein involved in photosynthesis and stress resistance (a) and with anti-mCherry antibody to detect Cry1Ab/c (b, 94 kDa). (A) GFP + Cry1Ab/c-cherry (27 kDa, lanes 1 and 4), 23KD-GFP + Cry1Ab/c-cherry (47 kDa, lanes 2 and 3); (B) Trx-GFP + Cry1Ab/c-cherry (48 kDa, lanes 1 and 5), THF1-GFP + Cry1Ab/c-cherry(59 kDa, lanes 2 and 6), G-GFP + Cry1Ab/c-cherry (45 kDa, lane 3 and 7), PSBP-GFP + Cry1Ab/c-cherry (50 kDa, lanes 4 and 8); (C) Rubisco-GFP + Cry1Ab/c-cherry (80 kDa); (D) CAMTAs-GFP + Cry1Ab/c-mCherry (117 kDa, lanes 1 and 6), DAHP-GFP + Cry1Ab/c-mCherry (86 kDa, lanes 2 and 5), HKMTs-GFP + Cry1Ab/c-cherry (102 kDa, lanes 3 and 4); (E) FREE1-GFP + Cry1Ab/c-cherry (116 kDa, lanes 1 and 4), KIN13A-GFP + Cry1Ab/c-cherry (117 kDa, lanes 10 and 11); and (F) E3s-GFP + Cry1Ab/c-cherry (83 kDa, lanes 14 and 17). The red asterisk indicates the target band.