TABLE 10.

Predicated functions of PL microbiota and their relative percentages (strength) in CD, SD and BD groups on the basis of COG analysis in the PICRUSt¹.

Predicted functions	Sequence hits in CD12	SD1 vs. CD1 (%)3	BD1 vs. CD1 (%)	Sequence hits in CD7	SD7 vs. CD7 (%)	BD7 vs. CD7 (%)
RNA processing and modification	12101	198.81**	159.68**	21626	98.62**	138.43**
[B] Chromatin structure and dynamics	32062	48.63**	33.28**	11077	104.62	315.35**
[C] Energy production and conversion	2795119	86.09**	60.44**	1829647	102.61	231.17*
[D] Cell cycle control, cell division, chromosome partitioning	418443	108.03**	76.88**	365147	101.35**	164.07**
[E] Amino acid transport and metabolism	4049675	78.49**	50.72**	2236313	102.74	225.01**
[F] Nucleotide transport and metabolism	942066	111.64**	75.23**	821300	102.60	172.87**
[G] Carbohydrate transport and metabolism	2090219	63.72**	46.11**	832215	103.18*	461.05**
[H] Coenzyme transport and metabolism	1883053	92.26**	64.38**	1332183	102.66	221.47**
[I] Lipid transport and metabolism	1737340	99.31**	68.16**	1322661	103.05*	186.28**
[J] Translation, ribosomal structure and biogenesis	2137244	113.36**	80.99**	1942789	102.69	173.76**
[K] Transcription	3160908	91.81**	61.26**	2135100	102.51**	226.71**
[L] Replication, recombination and repair	2141633	87.99**	64.14**	1470392	104.14**	259.25**
[M] Cell wall/membrane/envelope biogenesis	2280367	95.82**	73.68**	1696726	102.86	257.66**
[N] Cell motility	1155279	53.83**	47.45**	411624	101.51**	376.62**
[O] Post-translational modification, protein turnover, and chaperones	1749521	97.69**	71.49**	1355552	101.64**	183.21**
[P] Inorganic ion transport and metabolism	2447045	95.09**	66.12**	1795318	103.98**	168.70**
[Q] Secondary metabolites biosynthesis, transport, and catabolism	1102709	84.22**	55.62**	669183	103.00	236.36**
[R] General function prediction only	4745894	99.41**	68.19**	3581794	102.63*	230.71**
[S] Function unknown	3621424	93.51**	66.11**	2570393	102.89	193.14**
[T] Signal transduction mechanisms	2720944	57.67**	43.65**	1022903	102.47*	437.66**
[U] Intracellular trafficking, secretion, and vesicular transport	1101219	101.28**	80.43**	901110	102.07**	226.26**
[V] Defense mechanisms	603283	105.40**	83.14**	498363	103.51**	272.40**
[W] Extracellular structures	1850	2.27**	0.16**	1	100.00	30500.00**
[Y] Nuclear structure	0	0.00	0.00	0	0.00	0.00
[Z] Cytoskeleton	1047	25.41**	2.77**	7	542.86**	235685.71**

¹In the PICRUSTs, KEGG was not appropriate as it gave out analysis more related to humans, while COG analysis we found was more suitable for this work. ²Sequence hits (numbers) in CD1 and CD7 were directly cited from the outputs of COG analysis and treated as 100% percentiles in each predicted function at day 1 and 7, respectively. ³Percentages in each predicated function in SD and BD groups at day 1 or 7 were calculated as follows when compared to controls (CD1, CD7): the hit number in SD or BD group minus the hit number in CD on the same day, then the sum was divided by the hit number in CD of the same day and multiplied by 100 (%). The percentages thus obtained indicate the strength of a function in SD or BD group as compared to control (CD group). *Denotes significant difference as compared to control (p < 0.05). **Denotes extremely significant difference as compared to control (p < 0.01).