Figure 1.

Increased thymopoiesis in mice with general depletion of the AR (G-ARKO). (A,B) Thymus weight and cellularity in control (Pgk-Cre⁺; n = 10) and general androgen receptor knockout (G-ARKO; AR^flPgk-Cre⁺; n = 10) male mice. (C) Gating strategy for thymic subsets. (D–G) Number of CD4⁻CD8⁻ double negative thymocyte subsets (DN1-4, with different expression of CD44 and CD25 markers). (H–K) Total double negative (DN), double positive (DP; CD4⁺CD8⁺), and single positive (SP; CD4⁺ or CD8⁺) thymocytes in control (n = 10) and G-ARKO (n = 10) mice. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 (Mann-Whitney U-test); all bars indicate means; circles represent individual mice, error bars indicate SEM.