Figure 5.

Increased numbers of peripheral T cells and recent thymic emigrants (RTEs) in E-ARKO mice. (A) Gating strategy for CD4⁺ and CD8⁺ T cells and CD4⁺ and CD8⁺ RTEs. (B,C) Total numbers of CD4⁺ and CD8⁺ T cells in spleen from control (n = 18) and E-ARKO (n = 15) male mice. (D–G) Relative and total number of CD4⁺ and CD8⁺ RTEs (Qa2^loCD24^hi CD4⁺ or Qa2^loCD24^hi CD8⁺) in spleen from control (n = 18) and E-ARKO (n = 15) mice. (H,I) Relative number of CD4⁺ and CD8⁺ RTEs in lymph nodes (LN; pooled inguinal and para-aortic lymph nodes) from control (n = 11) and E-ARKO (n = 8) mice. (J,K) Relative number of CD4⁺ and CD8⁺ RTEs in blood from control (n = 10) and E-ARKO (n = 8) mice. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 (Mann-Whitney U-test); all bars indicate means; circles represent individual mice, error bars indicate SEM.