Table 2.
Details of the primary antibodies tested for immunohistochemistry with working dilutions and antigen retrieval methods.
| Antigen | Clone | Species | Dilution | Antigen retrieval | Manufacturer | Specificity |
|---|---|---|---|---|---|---|
| CD3 | F7.2.38 | Mouse | 1:100 | Microwavea | Dako, Glostrup, Denmark | T cell |
| Foxp3 | FJK-16S | Rat | 1:200 | Autoclaveb | eBioscience, California, USA | Regulatory T cell |
| CD20 | – | Rabbit | 1:800 | Microwave | Thermo Fisher, California, USA | B cell |
| Iba1 | – | Rabbit | 1:1,000 | Microwave | Wako, Osaka, Japan | Macrophage |
| CD68 | EBM11 | Mouse | 1:50 | Proteinase Kc | Dako | Exudate macrophage |
| CD163 | AM-3K | Mouse | 1:50 | Proteinase K | Trans Genic, Kumamoto, Japan | Kuppfer cell |
| CD204 | SRA-E5 | Mouse | 1:30 | Microwave | Trans Genic | Macrophage, Kupffer cell |
| CK19 | – | Rabbit | 1:200 | Microwave | Abcam, Cambridge, UK | Cholangiocyte |
CD, cluster of differentiation; Foxp3, forkhead box protein P3; Iba1, ionized calcium binding adaptor molecule 1; CK, cytokeratin.
a
Sodium citrate buffer pH 6.0 in microwave at 97°C for 15 min.
b
Sodium citrate buffer pH 6.0 in autoclave at 121°C for 20 min.
c
0.02% proteinase K at 37°C for 45 min.