Figure 5.

LITT increases endothelial cell transcytosis. (a) Representative transmission electron microscopy images are shown following intravenous HRP injection as in Figure 4A in sham and laser-treated brain on post-laser day 3 (n = 3 for each condition). HRP-filled vesicles within endothelial cells are highlighted (arrows). Scale bar = 100 nm. (B and C) Data represent mean ± SEM. Quantification of vesicular density (vesicles per μm) per length of luminal (B) and abluminal (C) membrane shows increased transcytosis in brain endothelial cells of laser-treated brain compared to sham-treated brain (n = 3 for each condition, 20 capillaries per animal, t-test, *P < .0001).