Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 23;11:593. doi: 10.3389/fphys.2020.00593

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Zhang, Zhang, Li, Ren, Deng, Yin, Gao, Pan and Niu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Isolation, morphology, and identification of hUCMSCs. The umbilical cord (UC) was cut into 10 cm pieces (A). The blood vessels in the UC were separated to obtain WJ (B). The morphology of primary cells cultured for 7 days (C). The morphology of cells after passaging (D). Lipid droplets were observed after culturing in adipogenic conditions for 14 days (E), but not in the control groups (F). Alizarin red staining showed mineralized nodules in cells grown under osteogenic conditions (G,I); cells in the control group were not stained (H,I). The third-generation cells were positive for CD90 (98.77%), CD73 (95.81%), and CD105 (97.36%) and negative for CD34 (0.07%) and CD45 (0.11%), as analyzed by flow cytometry (J).