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. 2020 Jun 23;11:593. doi: 10.3389/fphys.2020.00593

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Copyright © 2020 Zhang, Zhang, Li, Ren, Deng, Yin, Gao, Pan and Niu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell motility and adhesion ability of hUCMSCs treated with LE-TDM in comparison with hUCMSCs treated with regular media. A wound healing assay was performed, and the migration of the cells into the wound area was followed by microscopy at T = 24 h (A). Data are presented as the percentage of wound closure. Values are expressed as the mean ± SD (*P < 0.05) (B). Migrated hUCMSCs were visualized using crystal violet (C). The number of migrated cells was increased in the LE-TDM-treated group compared with that in the control group. Values are expressed as the mean ± SD (***P < 0.001) (D). LE-TDM affects hUCMSC adhesion. hUCMSCs attached to Fn-coated plates were visualized using crystal violet (E). The number of cells attached to Fn-coated plates was counted. Values are expressed as the mean ± SD (***P < 0.001) (F).