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. 2020 Jun 23;11:593. doi: 10.3389/fphys.2020.00593

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Copyright © 2020 Zhang, Zhang, Li, Ren, Deng, Yin, Gao, Pan and Niu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression profiles of HIF1A-AS2 in VC (cocultured V-hUCMSCs) and the association between HIF1A-AS2 and the HIF-1 signaling pathway in VC. HIF1A-AS2 expression in V and VC was analyzed by qRT-PCR. The levels of HIF1A-AS2 in VC were normalized to that in V (A). Relative HIF1A-AS2 expression in VC transfected with HIF1A-AS2 siRNAs (B). Relative mRNA expression of genes involved in the Hif-1 signaling pathway in HIF1A-AS2-deficient VC was analyzed by qRT-PCR. (C–E) The relative level of gene expression was normalized against β-actin, and the control was set to 1.0. Values are expressed as the mean ± SD (***P < 0.001). VEGFA, Hif-1α, and CD31 protein expression in HIF1A-AS2-deficient VC was assessed by western blot (F).