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. 2020 Jul 9;51:89. doi: 10.1186/s13567-020-00810-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Humoral and T-cell mediated immune response of pigs from different treatment groups. Average HI titers in pigs against H1N1 (A) and H3N2 (B) influenza virus strains between different treatment groups before challenge. The HI titers for pigs from each treatment group are shown as mean (bar) + SD (error line). The dash lines indicate the detection limit from 1:10 to 1:640. The number of IFN-γ specific H1 (C) and H3 (D) secreting cells in pigs from lymph nodes collected at necropsy are shown by treatment group. The bars show the mean count + SD (error line) of IFN-γ secreting cell spots per million loading cells for pigs from each treatment groups. The data points indicate the spot counts for samples collected from each individual pig. Both HI titers and ELISPOT counts were log transformed first and analyzed using a generalized linear mixed model and the Tukey test was used to perform multiple LSmeans comparisons. The asterisks denote the significant difference (P < 0.05) of HI titers or ELISPOT counts between groups. The dark blue, green and dark red bars and/or data points represent the pigs from the whole inactivated vaccine comparisons, negative control and live attenuated vaccine comparison groups, respectively.