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. 2020 Jul 2;11:1006. doi: 10.3389/fphar.2020.01006

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Copyright © 2020 Zhu, Wu, Zeng, Chen, Zheng, Ren and Ke

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RAP enhanced Dex-Ace-reduced TM cell autophagy and Dex-Ace-induced GIG-related protein expression at the 4th week. The relative protein expression levels of Beclin-1 and LC3-II/LC3-I ratio were upregulated, and p62 was downregulated in the Dex-Ace-treated group. RAP treatment further increased beclin-1 and LC3-II/LC3-I ratio, and downregulated p62 (A, B, C, D). Myocilin and a-actin were upregulated in the Dex-Ace-treated group and neutralized by RAP treatment (A, E, F). (n = 6; *, p < 0.05).