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. 2020 Jun 12;7(2):23. doi: 10.3390/jcdd7020023

Figure 8.

Figure 8

Right and left ventricular epicardial strains in normal and left heart hypoplasia chick embryos. (A) Representative video image of 10 μm diameter microspheres attached to the left ventricular (LV) epicardium of a stage 24 chick embryo. Epicardial strains were measured by tracking the motion of microspheres in triangular arrays. Scale bar = 50 μm. (B) Representative midventricular transverse sections for stage 27 normal (1.) and left-atrial ligated (LAL) (2.) chick embryos. Note the obvious increase in right ventricular (RV) and decrease in LV dimensions. Scale bar = 1 mm. (C) Representative developmental changes in strain–time curves for normal stage 21 chick ventricles; (D) stage 21 LAL chick ventricles; (E) stage 31 normal chick ventricles, and; (F) stage 31 LAL chick ventricles. Solid, broken, and dashed lines indicate epicardial circumferential, longitudinal, and shear strains, respectively. The x-axis is time (msec) and the y-axis is strain normalized to end-diastole. Note that from stage 21 to 31, the strain patterns change from isotropic to RV- and LV-specific anisotropic patterns and that LAL strains at stage 31 markedly differ from normal embryos. This was adapted with permission [134].