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Phylogenetic Tree. The evolutionary relationships of Orientia species detected outside of the Tsutsugamushi Triangle were compared with Orientia tsutsugamushi strains, Rickettsia species and other bacteria. The tree was constructed with the 560 bp rrs gene fragments using the Maximum Likelihood method and the Tamura–Nei model in MEGAX. The values of the bootstrap test (1000 replicates) were shown next to the branches.
