Figure 3.

Characterization of intestinal differentiation of the Caco-2 monolayer under different culture conditions. (a) Scanning electron microscope (SEM) images of the morphology of Caco-2 on chip under static, straight flow, and peristaltic flow conditions. (b) The ALP vitality of intestinal cells under distinct cultures were assessed on apical (AP) and basolateral (BL) sides, respectively. Results were obtained from triplicate independent experiments (N = 3). Data are shown as mean ± SD. ** p < 0.01. (c) Quantified analysis of the Caco-2 cell viability by cell counting kit-8 (CCK-8) kit. N = 3, * p < 0.05.