Figure 5.

Simulation of dynamic drug absorption and excretion profiles using fluorescein on a microfluidic system. (a) Schematic drawing for the administration of fluorescein into the upper channel of device. The control parameters include flow rate, fluorescein concentration, and administration time. (b) Quantification of fluorescein concentration diffused over the bottom channel by varying the flow rate (30, 60, 120 μL/h) in the upper channel at different time points. The fluorescein concentration (20 μg/mL), administration time (2 h), and flow rate in the bottom channel (1 μL/h) are constant. (c) Quantified analysis of fluorescein concentration with different flow rates (0.5, 1, 2 μL/h) in the bottom channel. The administration time (2 h), fluorescein concentration (20 μg/mL), and flow rate in the upper channel (60 μL/h) are constant. (d) Varying the fluorescein concentrations (10, 20, 40 μg/mL) with fixed administration time (2 h) and flow rates in the upper channel (60 μL/h) and bottom channel (1 μL/h). (e) Varying administration time (1, 2, 4 h) of fluorescein administration with fixed concentration and flow rates.