Figure 6.

Modeling PK profiles of anti-cancer drug 5-FU administration in the microfluidic system. (a) Schematic drawing of two regimens of drug administration on chip. In the PK profile regimen (Pk group), 20 μg/mL 5-FU exposed cells for 2 h was then withdrawn for 10 h, over a period of 12 h. In the constant profile regimen (Con group), 5-FU (20 μg/mL) was continuously infused into the channel and treated cells for 48 h. (b) Detection of 5-FU peaks by mass spectrometry (MS) after treating cells with 5-FU for 48 h. (c) Characterization of concentration-time curve after treatment of cells with 20 μg/mL 5-FU for 2 days.