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. 2020 Jun 19;17(12):4404. doi: 10.3390/ijerph17124404

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Morphology of human Schwann cell (hSC) in culture. hSCs were cultured, maintained and assumed a spindle, bipolar shape after 4 days. (B) Characterization of hSC through immunocytochemistry. Cultures were immunostained with antibodies against p75 NGFR, GFAP, MPZ, s100, MBP, MOG as indicated in the figure. hSCs do not express MOG. All cultures were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). Human Wharton jelly mesenchymal stem cells (hWJ-MSCs) were used as the negative control.