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The effect of javamide-II on IL-6 mRNA. PMA-differentiated THP-1 cells were treated with javamide-II (0. 1, 5, 15, 30 µM) followed by treatment with LPS for 4 h as described in “Materials and Methods”. After 4 h, total RNA was isolated and gene expression was determined using (RT)-PCR. Data are expressed as mean ± SD (n = 3). The asterisks (*) indicate significant difference from vehicle control (P < 0.002).
