Table 5.
Robustness data for green the RP-HPTLC and NP-HPTLC methods (n = 6).
| Conc. (ng/band) |
Mobile Phase Composition (ethanol:water:ammonia Solution) | Results | ||||
|---|---|---|---|---|---|---|
| Original | Used | Area ± SD | % RSD | Rf | ||
| Green RP-HPTLC method | ||||||
| 5.2:3.8:2 | +0.2 | 9614.8 ± 106.9 | 1.11 | 0.83 | ||
| 300 | 5:4:2 | 5:4:2 | 0.0 | 9589.8 ± 104.1 | 1.08 | 0.84 |
| 4.8:4.2:2 | −0.2 | 9454.3 ± 114.8 | 1.21 | 0.85 | ||
| NP-HPTLC method | ||||||
| Mobile phase composition (ethyl acetate: methanol: ammonia solution) | ||||||
| 5.2:3.8: 2 | +0.2 | 7374.6 ± 122.7 | 1.66 | 0.43 | ||
| 300 | 5:4:2 | 5:4:2 | 0.0 | 7298.4 ± 116.5 | 1.59 | 0.44 |
| 4.8:4.2:2 | −0.2 | 7153.7 ± 134.4 | 1.87 | 0.45 | ||