Table 4.

Primer sequences and target genes used for the detection of antimicrobial resistance determinants in resistant thermophilic Campylobacter spp. isolates and sialylated lipooligosaccharide (LOS) locus classes A, B and C in streptomycin-resistant C. jejuni isolates.

Primer	Type	Sequence (5’ - 3’)	Target	Amplicon Size (bp)	Annealing (°C )	Reference
GZgyrA5	A + S	ATT TTT AGC AAA GAT TCT GAT	QRDR region of gyrA	673	50	[15]
GZgyrA6		CCA TAA ATT ATT CCA CCT GT
TetO-FW	A	ACG GAR AGT TTA TTG TAT ACC	tetO	171	52	[103]
TetO-RV		TGG CGT ATC TAT AAT GTT GAC
TetO/W/O-F	A	ATC CAG ACA GCA GTG ACA TC	tetO/W/O	489	50	This study
TetO/W/O-R		ATG ATA GAC CGG AAA CAG GG
TetO/32/O-i-F	A	GAT ACA ATG AAT TTG GAG CG	tetO/32/O type I	545	48	This study
TetO/32/O-i-R		AAT TGT CTT TTG CAC TCC C
TetO/32/O-ii-F	A	CGG GCA GGT TTT TAAG ATT G	tetO/32/Oi type II	365	50	This study
TetO/32/O-ii-R		CTG TAT CAG CAA TCT CTG CG
F2-campy-23S	A + S	AAT TGA TGG GGT TAG CAT TAG C	Domain V of 23S rRNA	316	55	[121]
R2-campy-23S		CAA CAA TGG CTC ATA TAC AAC TTG
L4C-F	A + S	TTA TCC CTC TTT TGT AAT AGA TTC TAA	rplD	614	48	[61]
L4C-R		ATG AGT AAA GTA GTT GTT TTA AAT GAT
L22C-F	A + S	TTA GCT TTC CTT TTT CAC TGT TGC TTT	rplV	425	48	[61]
L22C-R		ATG AGT AAA GCA TTA ATT AAA TTC ATA AG
erm(B)-F	A	GGG CAT TTA ACG ACG AAA CTG G	ermB	421	52	[111]
erm(B)-R		CTG TGG TAT GGC GGG TAA GT
CmecoliF3	A + S	AATGTTTTAGCCGATACT	cmeABC	428	45	[28]
CmecoliR4		AACACCGCTTACTTGAGG
cst-II-F	A	ATG AAA AAA GTT ATT ATT GCT GGA AAT G	LOS locus class A/B	885/876	50	[122,123]
cst-II-R		TTA TTT TCC TTT GAA ATA ATG CTT TAT
orf14c-F	A	CAA CTT TGC AAA ATG ATT TTA TCT ATC ATT	LOS locus class C	995	50	[123]
orf14c-R		ATG CAA ATA CAA CAA AAC AAT TC

A, amplification; A + S, amplification and sequencing