Figure 2.

Protein-coding and lncRNA expression profile of S. japonicum throughout sexual maturation. (a) Multidimensional scaling (MDS) analyses of protein-coding and lncRNA gene expression together, detected by RNA-Seq in 48 samples of S. japonicum males (blue) and females (red) perfused from mice at 14 to 28 days post-infection. The number of days post-infection are indicated by the letters A to H, according to the legend on the right; three replicate samples for each day were analyzed. (b) Gene expression heatmap of protein-coding and lncRNA genes together (each in one line), across all 48 samples (each in one column) as indicated by the sample ID label at the bottom. Unsupervised clustering using the Euclidean distance was performed; expression of each gene is shown as the z-score (from −6 to 6), which is the number of standard deviations above (red) or below (blue) the mean expression value of that gene, across all RNA-Seq libraries; the z-score color scale is shown on the right. Samples from days 14 to 20 are labeled at the top as immature parasites (green), and from days 22 to 28 as mature parasites (purple). Males are labeled at the top in blue and females in red.