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Summary of the expression of recombinant deubiquitinating enzymes (DUBs) and the in vitro DUB assay. USP: ubiquitin specific protease; OTU: ovarian tumor domain containing protease; UCH: Ubiquitin C-terminal hydrolase; JAMM: JAB1/MPN/Mov34 metalloenzyme.
| Family (in Human Genome) | Expression Construct | Successfully Synthesized | Active DUBs | Inactive DUBs |
|---|---|---|---|---|
| USP (56) | 54 | 54 | 52 | 2 |
| OTU (17) | 16 | 16 | 14 | 2 |
| UCH (4) | 4 | 4 | 2 | 2 |
| Josephin (4) | 4 | 4 | 4 | 0 |
| JAMM (12) | 11 | 10 | 8 | 2 |
| Total (93) | 89 | 88 | 80 | 8 |
