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. 2020 Jun 8;11(6):577. doi: 10.3390/mi11060577

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Setup scheme and operational sequence for the iterative measurement of droplet sequences. Modules: A: syringe pump; B: valve module; C: void volume; D: droplet sequence storage module; E: photo-fluorimetric sensor. Operational sequence: (1) After setup initiation, the droplet sequence is in the upper part of the storage module; (2) The (A) syringe pump dispenses fluid and pushes the droplet sequence through the (E) sensor; The (C) void volume container is filled by the carrier medium; (3) The droplet sequence is in the lower part of the (D) storage module and the (B) valve block is switched to aspirate carrier medium from the (C) void volume back into the (A) syringe; (4) When the (A) syringe is refilled the (B) valve module is switched to this flow path; (5) The (A) syringe delivers fluid and pushes the droplet sequence from the lower part of the (D) storage module through the (E) sensor into the upper part of the (D) storage module; (6) The droplet sequence is in the upper part of the (D) storage module and the (B) valve block is switched to aspirate carrier medium from the (C) void volume back into the syringe; When the syringe is refilled the sequence restarts at (1).