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. 2020 Jun 8;11(6):577. doi: 10.3390/mi11060577

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Schematic view on the photo-fluorimeter core unit. The design allows the simultaneous recording of absorbance and fluorescence signals from a passing droplet. (A) Excitation laser diode; (B) fluorescence excitation filter; (C) photodiode for absorbance measurement; (D) Photosensor (avalanche diode) for the detection of fluorescence light; (E) fluorescence emission filter; (F) FEP-tube for guiding the droplet sequences; (G) optical beam forming elements. The excitation wavelength of the laser diode (A) and filters (B + E) must be adapted to the desired fluorescence wavelength.