Figure 9.

Analysis of the droplet composition accuracy regarding to the desired programmed composition. Two-dimensional program with stepwise varied compositions of two reporter dyes (C. red A and Orange G) A sequence of about 800 droplets was analyzed by two wavelength photometry. Absorbance of the two dyes (505 and 470 nm) were detected independently. (A) Syringe pump program for the generation of 2D concentration spaces in 11 concentration steps resulting in 121 concentration points; (B) measured droplet absorbance of reporter dyes; (C) segment composition accuracy based on the measured dye concentration combinations as normalized absorbance/absorbance plot; (D) detailed cutout of measured values; (C) data shows that the precision of the desired droplet composition is about ±2.5 and can be achieved with a deviation of about ±1.5%.