Figure 4.

Hemolysis study of selected conjugates in comparison to vancomycin. The hemolytic activity of selected conjugates was investigated via a hemoglobin release assay. The concentrations applied ranged between 1 and 900 µM. (A) V_V-modified conjugates are compared with vancomycin. (B) The same modification at the three different modification sites is compared with vancomycin. All tested conjugates showed no relevant hemolytic effect in target concentrations (below 2 µM). Data (% lysis relative to complete lysis by TRITON^®X-100) are shown as the mean of three independent experiments tested in duplicate.