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. 2020 May 26;6(2):19. doi: 10.3390/ncrna6020019

Figure 5.

Figure 5

FAK is a novel target of miR-204. (A) pmiR-LUC-FAK plasmids showing the seed region for miR-204 in the 3′UTR of wild type and mutated FAK sequences; (B) Firefly luciferase activity assays in MDA-MB-231 cells co-transfected with wild type and mutated pmiR-LUC-FAK plasmids and miR-204 mimics; (C) Western blot assays FAK protein in MDA-MB-231 cells treated with miR-204 mimics (lane 3), scramble (lane 2), and non-transfected control (lane 1); (D,E) Immunofluorescence and confocal microscopy assays using anti-FAK antibodies (Alexa-488, green channel), rhodamine-phalloidin (TRITC, red channel) for cytoskeleton staining, and DAPI for nuclear DNA staining (blue channel) in MDA-MB-231 cells treated with (E) DsiRNA HOTAIR and (D) non-transfected control. Experiments were performed three times by triplicate and data were expressed as mean ± S.D. ** p < 0.05.