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. 2020 May 11;9(2):14. doi: 10.3390/antib9020014

Figure 5.

Figure 5

MRB140 is specific for both native LL37 and cit-LL37 but does not recognize carb-LL37 nor merely citrullines or homocitrullines. (a) MRB140 was diluted in streptavidin ELISA plates coated with biotinylated native LL37 or cit-LL37 or control SCR LL37 peptide at the indicated dilutions, and ELISA tests were performed as in Material and methods. (b) MRB140 does not recognize carb-LL37 nor citrulline and homocitrulline themselves. MRB140 was diluted in ELISA plates coated with native LL37 or cit-LL37 or carb-LL37 peptide or with control carb-REV or cit-REV peptides. ELISA tests were performed as in Material and Methods. In both (a) and (b) results are expressed as the mean of triplicate cultures. Standard errors of the mean are indicated. Representative results form three independent experiments. p values by Student’s paired t-test (two-tailed) refer to difference between recognition of cit-LL37 as compared to all the other control peptides as indicated.