Skip to main content
. 2020 Jun 16;6(2):88. doi: 10.3390/jof6020088

Figure 2.

Figure 2

Alp1 induces ASM contraction. (a) Human ASM cells were cultured to confluence upon elastic polydimethylsiloxane (PDMS) NuSil® Gel-8100 (NuSil Silicone Technologies, CA) substrates prepared in 96-well plates as outlined in [35]. As the cells contract, they displace the substrate; based on the displacement of substrate-bound fluorescent beads relative to a cell-free image, and with knowledge of substrate stiffness and thickness, cellular contractile maps can be obtained. Such maps were obtained for ASM cells treated with either vehicle (average value of ASM contraction from map = 8.1 Pa) or Alp1 (average value of ASM contraction from map = 10 Pa). Scale bar = 100 µm. (b) Airway constriction (arrows) in human PCLS pre-treated with Alp1 or vehicle and stimulated with carbachol (CCh) as outlined in [18].